Detailed view for TcCLB.508257.80

Basic information

TDR Targets ID: 46312
Trypanosoma cruzi, WD domain, G-beta repeat, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1482 | Length (AA): 1203 | MW (Da): 133158 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
13 205 2h14 (A) 62 261 15.00 0 0.77 0.17 0.31
38 189 1erj (A) 445 622 17.00 0.00000000081 0.54 0.17 -0.42
476 821 1nr0 (A) 26 422 16.00 0.000001 0.89 0.19 0.52
1 396 3cfs (B) 11 402 15.00 0 0.71 0.195477 1.03
14 136 1gxr (A) 601 729 11.00 0.94 0.03 0.106844 0.41
15 118 3sfz (A) 643 753 13.00 0.81 0.12 0.147051 -0.06
65 144 5ams (A) 349 429 16.00 0 0.33 0.2468 -0.11
94 185 3dm0 (A) 1180 1262 29.00 0.26 1 0.364775 -0.08
97 152 3sfz (A) 1113 1164 40.00 0.23 0.56 0.16685 1.26
125 187 1jmx (B) 9 71 14.00 0 0.11 0.145669 0.46
986 1178 5cqs (A) 995 1262 19.00 0.0000061 0.36 0.177032 1.12
1000 1202 5cwi (A) 2 202 15.00 0.0037 0.07 0.339345 -0.81
1038 1136 3kd7 (A) 8 107 9.00 0.091 0.07 0.158894 -0.17

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_143078)

Species Accession Gene Product
Leishmania braziliensis LbrM.20.3390   hypothetical protein, conserved
Leishmania donovani LdBPK_343590.1   hypothetical protein, conserved
Leishmania infantum LinJ.34.3590   hypothetical protein, conserved
Leishmania major LmjF.34.3790   hypothetical protein, conserved
Leishmania mexicana LmxM.33.3790   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.4.770   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.960   WD domain, G-beta repeat, putative
Trypanosoma congolense TcIL3000_4_580   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506405.4   WD domain, G-beta repeat, putative
Trypanosoma cruzi TcCLB.409101.9   WD domain, G-beta repeat, putative
Trypanosoma cruzi TcCLB.508257.80   WD domain, G-beta repeat, putative

Essentiality

TcCLB.508257.80 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.960 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.960 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TcCLB.508257.80 (Trypanosoma cruzi), WD domain, G-beta repeat, putative
Title for this comment
Comment